Metabolism and cellular organisation
Another characteristic of life is metabolism. All living organisms are made of chemicals. These chemicals, small and big, belonging to various classes, sizes, functions, etc., are constantly being made and changed into some other biomolecules. These conversions are chemical reactions or metabolic reactions. There are thousands of metabolic reactions occurring simultaneously inside all living organisms, be they unicellular or multicellular. All plants, animals, fungi and microbes exhibit metabolism. The sum total of all the chemical reactions occurring in our body is metabolism. No non-living object exhibits metabolism. Metabolic reactions can be demonstrated outside the body in cell-free systems. An isolated metabolic reaction(s) outside the body of an organism, performed in a test tube is neither living nor non-living. Hence, while metabolism is a defining feature of all living organisms without exception, isolated metabolic reactions in vitro are not living things but surely living reactions. Hence, cellular organisation of the body is the defining feature of life forms.